|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues T(298) K Y L K E N F S P W I Q Q H(312) of the long form of human Bcl-G.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-4209 detects the long form of BCL-G in in human samples.
PA1-4209 has been successfully used in Western blot procedures.
The PA1-4209 immunogen is a synthetic peptide corresponding to residues T(298) K Y L K E N F S P W I Q Q H(312) of the long form of human Bcl-G.
Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human BCL-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Candidate tumour suppressor Fau regulates apoptosis in human cells: an essential role for Bcl-G.
PA1-4209 was used in western blot to study the pro-apoptotic mechanism of candidate tumor suppressor Fau in human cells
|Pickard MR,Mourtada-Maarabouni M,Williams GT||Biochimica et biophysica acta (1812:1146)||2011|
Apoptosis regulators Fau and Bcl-G are down-regulated in prostate cancer.
PA1-4209 was used in western blot to study the expression of Fau and Bcl-G in prostate cancer
|Pickard MR,Edwards SE,Cooper CS,Williams GT||The Prostate (70:1513)||2010|