|Tested species reactivity||Human, Mouse, Pig, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. This amino acid sequence is shared by human and murine bcl-X protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice.
Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.
Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain that is well-conserved among members of the Bcl-2 family. This family of proteins is an important regulator of apoptosis. Bcl xL forms heterodimers with BAX, BAK, and Bcl2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl xL is phosphorylated on many sites including serine 62, a critical site for Bcl xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 is thought to be mediated by Jun N-terminal stress kinase (JNK) signaling - negatively regulates the anti-apoptotic function of Bcl xL and controls the growth of neoplastic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study.
MA5-14011 was used in immunohistochemistry - paraffin section to identify prognostic biomarkers for complex karyotype soft tissue sarcoma
|Lahat G,Tuvin D,Wei C,Wang WL,Pollock RE,Anaya DA,Bekele BN,Corely L,Lazar AJ,Pisters PW,Lev D||Annals of oncology : official journal of the European Society for Medical Oncology (21:1112)||2010|
Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
MA5-14011 was used in western blot to examine the anti-apoptotic role of Bcl-xL and myeloid cell leukaemia-1 in colorectal cancer cells
|Schulze-Bergkamen H,Ehrenberg R,Hickmann L,Vick B,Urbanik T,Schimanski CC,Berger MR,Schad A,Weber A,Heeger S,Galle PR,Moehler M||World journal of gastroenterology (14:3829)||2008|
Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.
MA5-14011 was used in western blot to study the role of Bcl-2 and mitochrondria in the neuroprotective effects of minocycline
|Wang J,Wei Q,Wang CY,Hill WD,Hess DC,Dong Z||The Journal of biological chemistry (279:19948)||2004|
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
MA5-14011 was used in immunohistochemistry to investigate the role of Epstein-Barr virus infection in the immune response of non-monomorphic post-transplant lymphoproliferative disorders
|Shaknovich R,Basso K,Bhagat G,Mansukhani M,Hatzivassiliou G,Murty VV,Buettner M,Niedobitek G,Alobeid B,Cattoretti G||Haematologica (91:1313)||2006|
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
MA5-14011 was used in immunohistochemistry to investigate cell death in osteoarthritic cartilage and the role of caspase-3 during this process
|Sharif M,Whitehouse A,Sharman P,Perry M,Adams M||Arthritis and rheumatism (50:507)||2004|
Bcl-x(S) induces an NGF-inhibitable cytochrome c release.
MA5-14011 was used in immunocytochemistry to elucidate the role of Bcl-x(S) in nerve growth factor-inhibitable apoptosis
|Lindenboim L,Schlipf S,Kaufmann T,Borner C,Stein R||Experimental cell research (297:392)||2004|
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
MA5-14011 was used in immunohistochemistry to study the role of various bcl-2 family molecules in the regulation of apoptosis and the progression of urothelial cancer.
|Korkolopoulou P,Lazaris ACh,Konstantinidou AE,Kavantzas N,Patsouris E,Christodoulou P,Thomas-Tsagli E,Davaris P||European urology (41:274)||2002|