|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 3-14 of human and mouse Bcl-XL and Bcl-XS.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
Suggested positive control: antigen standard for BCL2L1 (transient overexpression lysate), Jurkat, MOLT-4, or Rh-30.
The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. The balance of expression between these genes may determine the death or survival of a cell. In addition to its anti-apoptotic effect, Bcl-X has two isoforms, resulting from alternative splicing. Bcl-XL (long) is a 241 amino acid protein (27 kD) and Bcl-XS is a 178 amino acid protein with predicted molecular weight of 19.5 kD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.