|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminus of human Bcl-x protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma.
BCL-X has two isoforms, BCL-XL (long) a 241 amino acid protein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the BCL-2 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator Bcl-X; Bcl-2-like protein 1; Bcl-X; bcl-xL; BCL-XL/S; bcl-xS; Bcl2-L-1; BCL2-like 1; BCL2L; BCLX; BCLXL; BCLXS; PPP1R52; protein phosphatase 1; protein phosphatase 1, regulatory subunit 52; regulatory subunit 52
Gene Aliases: Bcl-X; BCL-XL/S; BCL2L; BCL2L1; BCLX; PPP1R52
UniProt ID: (Human) Q07817
Entrez Gene ID: (Human) 598
Molecular Function: signaling molecule
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