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Immunofluorescent analysis of BCL10 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a BCL10 polyclonal antibody (Product # PA5-34800) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 233 of BCL10 (Uniprot ID#O95999)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34800 targets BCL10 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34800 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 233 of BCL10 (Uniprot ID#O95999).
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B-cell CLL/lymphoma 10; B-cell leukemia/lymphoma 10; B-cell lymphoma/leukemia 10; BCL-10; c-E10; CARD containing molecule enhancing NF-kB; CARD-containing apoptotic signaling protein; CARD-containing molecule enhancing NF-kappa-B; CARD-containing proapoptotic protein; CARD-like apoptotic protein; CARMEN; caspase-recruiting domain-containing protein; cCARMEN; CED-3/ICH-1 prodomain homologous E10-like regulator; cellular homolog of vCARMEN; cellular-E10; CIPER; CLAP; hCLAP; mammalian CARD-containing adapter molecule E10; mE10
AI132454; BCL-10; BCL10; c-E10; C81403; CARMEN; cE10; CIPER; CLAP; IMD37; ME10