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Invitrogen
BDH1 Recombinant Rabbit Monoclonal Antibody (PSH14-69)
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FIGURE: 1 / 12
BDH1 Antibody (MA570036) in ICC/IF
Immunocytochemistry analysis of BDH1 in HepG2 cells. Samples were incubated with BDH1 monoclonal antibody (Product # MA570036) using a dilution of 1:200 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was ... View More
Product Details
MA570036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH14-69
Immunogen
Recombinant protein within human BDH1 aa 1-343.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: HepG2 cell lysate, HT-29 cell lysate, COLO205 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, human colon tissue, human kidney tissue, rat spleen tissue.
Subcellular location: Mitochondrion inner membrane, Mitochondrion matrix.
Target Information
This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); 3-hydroxybutyrate dehydrogenase, type 1; b-hydroxybutyrate dehydrogenase; b-OHBDH; BDH; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; epididymis secretory sperm binding protein; MGC2723; MGC4347; MGC9788; Short chain dehydrogenase/reductase family 9C member 1
Gene Aliases: 2310032J20Rik; AI327223; BDH; BDH1; SDR9C1
UniProt ID: (Human) Q02338, (Rat) P29147, (Mouse) Q80XN0
Entrez Gene ID: (Human) 622, (Rat) 117099, (Mouse) 71911
lipid metabolic process
steroid metabolic process
liver development
brain development
response to nutrient
response to toxic substance
response to hormone
response to estradiol
response to insulin
response to drug
response to starvation
response to ethanol
response to cadmium ion
response to corticosterone
oxidation-reduction process
response to growth hormone
adipose tissue development
metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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