HeLa cells were lysed 96 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non- Targeting Pool (Lane 2), or 100nM ON-TARGETplus BID siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding the cleavage site of human BID|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
82321 targets BID in Western blot applications and shows reactivity with human samples. BID has a predicted molecular weight of approximately 23 kDa.
Store at -20°C and do not aliquot.
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.