Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1-2 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Mouse / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
23F7 |
Immunogen |
Residues 5-195 from human recombinant protein expressed in bacteria |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_10985736 |
MA1-13043 detects BID from human and mouse samples.
MA1-13043 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects a 22 kDa protein corresponding to human BID.
The MA1-13043 antigen is a recombinant protein corresponding to residues 5-195 of human BID.
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID
Gene Aliases: 2700049M22Rik; AI875481; AU022477; BID; FP497
UniProt ID: (Human) P55957, (Mouse) P70444
Entrez Gene ID: (Human) 637, (Mouse) 12122
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