Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
3C5 |
Immunogen |
Purified recombinant full length human BID protein expressed in E. Coli |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites |
Contains |
0.03% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2538506 |
MA5-17034 targets BID in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-17034 immunogen is purified recombinant fragment of human BID expressed in E. Coli.
MA5-17034 detects BID which has a predicted molecular weight of approximately 22kDa.
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID
Gene Aliases: BID; FP497
UniProt ID: (Human) P55957
Entrez Gene ID: (Human) 637
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