Invitrogen

BID Monoclonal Antibody (3C5)

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

BID Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HeLa cells using BID monoclonal antibody (Product # MA5-17034) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

BID Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and tonsil tissues (right) using BID monoclonal antibody (Product # MA5-17034) followed with DAB staining.

BID Antibody in Western Blot (WB) — Knockdown of BID was achieved by transfecting Hep G2 cells with BID specific siRNAs (Silencer® select Product # s1986). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the BID knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with BID Monoclonal Antibody (Product # MA5-17034, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to BID.

BID Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HeLa cells using BID monoclonal antibody (Product # MA5-17034) (green) and negative control (purple).

BID Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HepG2 cells were transfected with BID siRNA and reduction of signal was observed in Western Blot using BID Monoclonal Antibody (Product # MA5-17034). {KD}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:200 - 1:400

Immunocytochemistry (ICC)

1:200 - 1:1000

Immunofluorescence (IF)

1:200 - 1:1000

Immunohistochemistry (IHC)

1:200 - 1:1000

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species

Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C5

Immunogen

Purified recombinant full length human BID protein expressed in E. Coli

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2538506

Product Specific Information

MA5-17034 targets BID in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17034 immunogen is purified recombinant fragment of human BID expressed in E. Coli.

MA5-17034 detects BID which has a predicted molecular weight of approximately 22kDa.

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID

Gene Aliases: BID; FP497

UniProt ID: (Human) P55957

Entrez Gene ID: (Human) 637

Function(s)