|Tested species reactivity||Mouse|
|Host / Isotype||Hamster / IgG|
|Immunogen||A complex of mouse BIM and Bcl-XL produced in insect cells.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (FACS)||Neat-1:10|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Bcl-2 (B-cell lymphoma) and close homologues promote cell survival while many of its relatives lead to apoptotic cell death. The Bcl-2 homology 3 (BH3) domain is a potent death domain, and a novel BH3 domain containing protein, termed Bim or BOD (Bcl-2-related Ovarian Death Gene), has recently been cloned. Bim possesses a hydrophobic C-terminus and is localized to the intracytoplasmic membranes. Bcl-2 binds to Bim and modulates its death function, while Bcl-2 mutants are unable to do either.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.