Invitrogen

BIN3 Monoclonal Antibody (OTI5E3)

View all (22) BIN3 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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BIN3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of BIN3 in COS7 cells. Cells were transfected with a plasmid overexpressing BIN3 and probed with a BIN3 monoclonal antibody (Product # MA5-25302).

BIN3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a BIN3 monoclonal antibody (Product # MA5-25302).

BIN3 Antibody in Western Blot (WB) — Western blot analysis of BIN3 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with BIN3 (Product # MA5-25302) monoclonal antibody.

Product Details

MA5-25302

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Dog, Human, Non-human primate, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5E3
Immunogen	Full length human recombinant protein of BIN3 produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.71 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2723987

Target Information

Involved in cytokinesis and septation where it has a role in the localization of F-actin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bridging integrator 3

Gene Aliases: BIN3

UniProt ID: (Human) Q9NQY0, (Rat) Q68FW8

Entrez Gene ID: (Dog) 608969, (Human) 55909, (Rat) 361065

Function(s)

cytokinesis barrier septum assembly actin filament organization protein localization unidimensional cell growth regulation of lamellipodium assembly myoblast migration involved in skeletal muscle regeneration skeletal muscle fiber development skeletal muscle tissue regeneration

Process(es)

protein binding cytoskeletal adaptor activity

BIN3 Monoclonal Antibody (OTI5E3)

Product Details

MA5-25302

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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