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Immunofluorescent analysis of A549 cells using a BLZF1 polyclonal antibody (Product # PA5-26605). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a BLZF1 polyclonal antibody (Product # PA5-26605) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 156-184 amino acids from the central region of human BLZF1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cytoplasmic protein; GOLGIN-45; JEM-1s; JEM-1short protein; JEM1; p45 basic leucine-zipper nuclear factor
BLZF1; GOLGIN-45; JEM-1; JEM-1s; JEM1