NeoBiotechnologies

BMI1 Monoclonal Antibody (BMI1, 2689)

View all (43) BMI-1 antibodies

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Protocols

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BMI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human colon carcinoma using BMI1 Monoclonal Antibody (Product # 648-MSM1-P1ABX).

BMI1 Antibody in Western Blot (WB) — Western Blot analysis of Human 293T cell lysate using BMI1 Monoclonal Antibody (Product # 648-MSM1-P1ABX).

BMI1 Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins BMI1 Monoclonal Antibody (Product # 648-MSM1-P1ABX). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

BMI1 Antibody — SDS-PAGE Analyis Purified BMI1 Monoclonal Antibody (Product # 648-MSM1-P1ABX). Confirmation of Purity and Integrity of Antibody.

Product Details

648-MSM1-P1ABX

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	BMI1, 2689
Immunogen	Recombinant fragment of human BMI1 protein (around aa 142-326) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20°C or -80°C if preferred

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

Gene Aliases: BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51

UniProt ID: (Human) P35226

Entrez Gene ID: (Human) 648

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated segment specification regulation of gene expression covalent chromatin modification protein sumoylation hemopoiesis negative regulation of gene expression, epigenetic positive regulation of fibroblast proliferation positive regulation of ubiquitin-protein transferase activity

Process(es)

protein binding zinc ion binding RING-like zinc finger domain binding promoter-specific chromatin binding