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Product Details
MA5-23915
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1 µg/mL | - |
Neutralization (Neu) |
0.15-0.9 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
462732 |
Immunogen |
CHO-derived recombinant human BMP-10 Asn317-Arg424 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.5 mg/mL |
Purification |
Protein A/G |
Storage buffer |
PBS with 5% trehalose |
Contains |
No Preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2609916 |
Product Specific Information
In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-beta superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation. It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes. BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BMP; BMP-10; Bone morphogenetic protein; Bone morphogenetic protein 10
Gene Aliases: BMP10
UniProt ID: (Human) O95393, (Mouse) Q9R229
Entrez Gene ID: (Human) 27302, (Mouse) 12154
Molecular Function:
growth factor
cell adhesion
adult heart development
negative regulation of endothelial cell migration
positive regulation of cardiac muscle hypertrophy
negative regulation of cardiac muscle hypertrophy
positive regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of cell growth
negative regulation of cell migration
BMP signaling pathway
activin receptor signaling pathway
regulation of apoptotic process
regulation of MAPK cascade
sarcomere organization
positive regulation of transcription, DNA-templated
atrial cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
ventricular cardiac muscle cell development
regulation of cardiac muscle contraction
cardiac muscle cell proliferation
positive regulation of cardiac muscle cell proliferation
positive regulation of sarcomere organization
heart trabecula formation
pathway-restricted SMAD protein phosphorylation
SMAD protein signal transduction
positive regulation of cartilage development
regulation of cardiac muscle hypertrophy in response to stress
positive regulation of cell proliferation involved in heart morphogenesis
multicellular organism development
heart development
positive regulation of gene expression
growth
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