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Western blot detection of BNIP3 in extracts of 293T cells transfected with human BNIP3. Samples were separated on SDS-PAGE and probed using a BNIP3 monoclonal antibody (Product # MA1-24688). The antibody was developed with AlkPh APA mouse Fab Ads Hu, Bov, Hor and an NBT/BCIP substrate.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant fragment corresponding to residues 1-163 of human BNIP3.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-24688 detects BNIP3 from human samples.
MA1-24688 has been successfully used in immunoprecipitation, immunocytochemistry, ELISA and Western blot procedures. Positive Control: 293T cells transfected with BNIP3.
The MA1-24688 immunogen is a recombinant fragment corresponding to residues 1-163 of human BNIP3. This antibody recognizes an epitope of residues 112-124 from human BNIP3.
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular and immunohistochemical characterization of B-cell lymphoma-2-negative follicular lymphomas.
MA1-24688 was used in immunohistochemistry to study B-cell lymphoma-negative follicular lymphomas at the molecular and immunohistochemical levels
|Hoeller S,Bihl MP,Zihler D,Cogliatti S,Ponzoni M,Zettl A,Went P,Foerster A,Hirschmann P,Tzankov A,Dirnhofer S||Human pathology (43:405)||2012|
BCL2/adenovirus E1B 19kDa interacting protein 3; NIP3