|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, k|
|Immunogen||Protein fragment expressed in E. coli corresponding to amino acids 341-748.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23162 detects BRCA1 in Human and Mouse samples.
MA1-23162 has been successfully used in Immunohistochemistry, Immunoprecipitation and Western Blot procedures. For immunohistochemistry (paraffin), perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1M citrate buffer at 95°C in water bath for 20 minutes). Avoid nonspecific binding by incubation in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.
MA1-23162 immunogen corresponds to Protein fragment expressed in E. coli corresponding to amino acids 341-748..
BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.