Bethyl Laboratories

BRCA1 Recombinant Rabbit Monoclonal Antibody (BLR319M)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

A700-319

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Flow Cytometry (Flow)

0.05 µL/1x10^6 cells
-

Immunoprecipitation (IP)

6 µL/mg
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR319M

Immunogen

Recombinant antibody was purified from cell culture supernatant. Immunogen was a peptide representing a region between residue 500 and 550 of mouse Breast cancer type 1 susceptibility protein (aka BRCA1) using the numbering given in entry NP_033894.3 (Gene ID 12189).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 6.8-7.4

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: brca 1; brca-1; breast cancer 1 early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein homolog; BROVCA12; Delta772-3095; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; RING-type E3 ubiquitin transferase BRCA1

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Gene Aliases: Brca1

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UniProt ID: (Mouse) P48754

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Entrez Gene ID: (Mouse) 12189

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Function(s)
DNA binding damaged DNA binding RNA binding ubiquitin-protein transferase activity zinc ion binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity enzyme binding ubiquitin protein ligase binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity transcription regulatory region DNA binding metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme
Process(es)
double-strand break repair via homologous recombination DNA replication DNA repair postreplication repair double-strand break repair DNA recombination regulation of gene expression by genetic imprinting lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process cellular response to DNA damage stimulus cell cycle chromosome segregation centrosome cycle intrinsic apoptotic signaling pathway in response to DNA damage dosage compensation by inactivation of X chromosome response to ionizing radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression protein ubiquitination chromosome breakage positive regulation of protein ubiquitination G2 DNA damage checkpoint positive regulation of histone acetylation negative regulation of histone acetylation regulation of cell proliferation chordate embryonic development response to estrogen regulation of DNA methylation negative regulation of fatty acid biosynthetic process positive regulation of DNA repair positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter centrosome duplication positive regulation of histone H3-K4 methylation negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation positive regulation of histone H3-K9 methylation protein autoubiquitination positive regulation of histone H4-K20 methylation positive regulation of cell cycle arrest cellular response to tumor necrosis factor cellular response to indole-3-methanol protein K6-linked ubiquitination negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of reactive oxygen species metabolic process positive regulation of histone H3-K9 acetylation positive regulation of histone H4-K16 acetylation
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