Active Motif

BRD4 Polyclonal Antibody

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BRD4 Antibody in Western Blot (WB) — BRD4 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (30 µg) probed with BRD4 antibody (pAb) at a dilution of 1:500.

BRD4 Antibody in ChIP assay (ChIP) — BRD4 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 10 µg of chromatin from TNF-alpha treated HT-29 colorectal adenocarcinoma cells and 5 µl BRD4 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Product Details

39910

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:1,000	-
ChIP assay (ChIP)	5-10 µL	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication
ChIP-sequencing (ChIP-Seq)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	This BRD4 antibody was raised against a peptide within the C-terminal region of human BRD4.
Conjugate	Unconjugated
Form	Liquid
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhancement of BDNF Expression and Memory by HDAC Inhibition Requires BET Bromodomain Reader Proteins.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sartor GC,Malvezzi AM,Kumar A,Andrade NS,Wiedner HJ,Vilca SJ,Janczura KJ,Bagheri A,Al-Ali H,Powell SK,Brown PT,Volmar CH,Foster TC,Zeier Z,Wahlestedt C

Research topic: Neurobiology

Wed Jan 23 00:00:00 EST 2019

Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sartor GC,Powell SK,Brothers SP,Wahlestedt C

Research topic: Neurobiology, Drug Addiction

Wed Nov 11 00:00:00 EST 2015

Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sartor GC,Powell SK,Brothers SP,Wahlestedt C

Research topic: Neurobiology, Drug Addiction

Wed Nov 11 00:00:00 EST 2015

The oncogenic BRD4-NUT chromatin regulator drives aberrant transcription within large topological domains.

Genes & development

Alekseyenko AA,Walsh EM,Wang X,Grayson AR,Hsi PT,Kharchenko PV,Kuroda MI,French CA

Research topic: Squamous cell cancer

Wed Jul 15 00:00:00 EDT 2015

The elongation complex components BRD4 and MLLT3/AF9 are transcriptional coactivators of nuclear retinoid receptors.

PloS one

Flajollet S,Rachez C,Ploton M,Schulz C,Gallais R,Métivier R,Pawlak M,Leray A,Issulahi AA,Héliot L,Staels B,Salbert G,Lefebvre P

Research topic: Transcription Regulation

Tue Feb 11 00:00:00 EST 2014

The elongation complex components BRD4 and MLLT3/AF9 are transcriptional coactivators of nuclear retinoid receptors.

PloS one

Flajollet S,Rachez C,Ploton M,Schulz C,Gallais R,Métivier R,Pawlak M,Leray A,Issulahi AA,Héliot L,Staels B,Salbert G,Lefebvre P

Research topic: Transcription Regulation

Tue Feb 11 00:00:00 EST 2014

Bioinformatics

Protein Aliases: BRD4-NUT FUSION; bromodomain-containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; chromosome-associated protein; fsh-like protein; MCAP; Mitotic chromosome-associated protein; Protein HUNK1

Gene Aliases: BRD4; HUNK1; Mcap