Immunoprecipitation analysis using Anti-BRD4 Recombinant Mouse Monoclonal Antibody (Cat. no. 730007) followed by western blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged BRD4 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation. Because detection utilizes an anti-flag antibody, the presence of multiple bands suggests multiple forms of flag-tagged BRD4 protein rather than cross-reactivity of the Anti-BRD4 Recombinant Mouse Monoclonal Antibody.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||333-460 of 1362 of BRD4 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730007 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|
BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors.
730007 was used in immunocytochemistry to evaluate the BioID proximity-based biotin labeling technique for the identification of novel c-MYC interacting partners in cultured cells and xenograft tumors
|Dingar D,Kalkat M,Chan PK,Srikumar T,Bailey SD,Tu WB,Coyaud E,Ponzielli R,Kolyar M,Jurisica I,Huang A,Lupien M,Penn LZ,Raught B||Journal of proteomics (118:95)||2015|