Invitrogen

BRD8 Recombinant Rabbit Monoclonal Antibody (1H3L15)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (18) BRD8 antibodies

Datasheet

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Datasheet

Questions & Answers

Product Image Gallery

BRD8 Antibody in Western Blot (WB) — Western blot analysis of BRD8 was performed by loading 100 ng of whole cell lysates from Control (Lane 1 and 2) and BRD8 Knock down cells (lane 3 and 4) in RIPA Lysis and Extraction buffer (Product # 89900) and Page Ruler Plus Protein Ladder (Product # 26619) onto a 30% Acrylamide/Bis gel. Proteins were transferred to membrane and blocked in 5% milk for one hour at room temperature. BRD8 was detected at approximately 140 kDa using a BRD8 recombinant monoclonal antibody (Product # 702872) at a dilution of 1:500 in 5% milk overnight at 4°C on a rocking platform, followed by a goat anti-rabit IgG-HRP secondary antibody at a dilution of 1:5,000 for one hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076) and the myECL Imager (Product # 62236). Data courtesy of Wei Xu’s laboratory at the University of Wisconsin.

BRD8 Antibody in Immunofluorescence (IF) — For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous BRD8 using Anti-BRD8 Recombinant Rabbit Monoclonal Antibody (Product # 702872, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BRD8 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of BRD8. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

BRD8 Antibody in ChIP assay (ChIP) — Chromatin Immunoprecipitation (ChIP) assay of endogenous BRD8 protein using Anti-BRD8 Antibody: ChIP was performed using Anti-BRD8 Recombinant Rabbit Monoclonal Antibody (Product # 702872, 5 µg) on sheared chromatin from 2 million HCT116 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to MMP13 and MMP2 transcriptional start site, GAPDH promoter and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

BRD8 Antibody in Knockout — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. Loss of signal was observed for target protein in BRD8 knockdown (KD) cell line using Anti-BRD8 recombinant monoclonal antibody (Product # 702872). {KO}

BRD8 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HCT 116 cells were transfected with BRD8 siRNA and a decrease in signal intensity was observed in western blot application using Anti-BRD8 Recombinant Rabbit Monoclonal Antibody (Product # 702872). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

ChIP assay (ChIP)

2.5 µg/million cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

1H3L15

Immunogen

Protein corresponding to human BRD8 [aa1-aa200]

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2848219

Product Specific Information

This antibody is predicted to react with Mouse, Bovine.

Target Information

Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bromodomain-containing protein 8; p120; Skeletal muscle abundant protein; Skeletal muscle abundant protein 2; Thyroid hormone receptor coactivating protein of 120 kDa; TrCP120

Gene Aliases: BRD8; p120; SMAP; SMAP2

UniProt ID: (Human) Q9H0E9

Entrez Gene ID: (Human) 10902

Function(s)

transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter signal transduction cell surface receptor signaling pathway intracellular receptor signaling pathway regulation of growth histone H4 acetylation histone H2A acetylation

Process(es)

transcription factor activity, sequence-specific DNA binding thyroid hormone receptor activity