Invitrogen

BRK Monoclonal Antibody (2H12B8)

View all (18) BRK antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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BRK Antibody in Western Blot (WB) — Western blot was performed using Anti-BRK Monoclonal Antibody (2H12B8) (Product # MA5-15328) and a 48kDa band corresponding to BRK was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of Caco-2 (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), A549 (Lane 4), PANC-1 (Lane 5), and HepG2 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

MA5-15328

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2H12B8

Immunogen

Purified recombinant fragment of human PTK6 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_10986428

Product Specific Information

MA5-15328 targets PTK6 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15328 immunogen is purified recombinant fragment of human PTK6 expressed in E. Coli.

.

MA5-15328 detects PTK6 which has a predicted molecular weight of approximately 52kDa.

Target Information

Brk is 80% identity to Sik (Src-related intestinal kinase) and exhibits the features of a novel non-receptor tyrosine kinase, including amino terminal SH3 and SH2 domains. Low levels of Brk transcripts have been identified in some human breast tumor cell lines but not in normal breast tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ALT-PTK6; Breast tumor kinase; deltam5; FLJ42088; Protein-tyrosine kinase 6; protein-tyrosine kinase BRK; PTK6 protein tyrosine kinase 6; Tyrosine-protein kinase BRK

Gene Aliases: BRK; PTK6

UniProt ID: (Human) Q13882

Entrez Gene ID: (Human) 5753

Molecular Function: protein-binding activity modulator scaffold/adaptor protein