Western blot analysis of BSA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using a BSA polyclonal antibody (Product # PA5-23403) at 0.05 ug/ml. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Bovine|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.02-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Bovine serum albumin/bovine albumin/BSA is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. It is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Structural basis for biofilm formation via the Vibrio cholerae matrix protein RbmA.
PA5-23403 was used in western blot to study the role of RbmA in biofilm formation
|Giglio KM,Fong JC,Yildiz FH,Sondermann H||Journal of bacteriology (195:3277)||2013|