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Product Details
200-301-900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
14H5
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human BUB1 protein. The product was purified from tissue culture supernatant by chromatography. This antibody reacts with BUB1 from human cells. No reactivity is seen for homologues from Xenopus or Kangaroo Rat. Reactivity against homologues from other sources is not known.
Target Information
BUB1A is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
Gene Aliases: BUB1; BUB1A; BUB1L; hBUB1
UniProt ID: (Human) O43683
Entrez Gene ID: (Human) 699
protein phosphorylation
apoptotic process
sister chromatid cohesion
regulation of sister chromatid cohesion
mitotic nuclear division
mitotic cell cycle checkpoint
mitotic spindle assembly checkpoint
cell proliferation
viral process
cell division
regulation of chromosome segregation
spindle assembly checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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