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|Tested species reactivity||Mouse|
|Host / Isotype||Sheep / IgG|
|Immunogen||Recombinant protein corresponding to residues 883-1052 of mouse Bub1b.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is mitotic NIH-3T3 cells.
Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 beta; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; SSK1
AU045529; Bub1b; BUBR1; Mad3l