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Western blot analysis of Bacillus anthracis Protective Antigen in using a Bacillus anthracis Protective Antigen monoclonal antibody (Product # MA1-21675, Lane 1) and alternate antibodies (MA1-21678, Lane 2, and MA1-21679, Lane 3). Proteins were separated on 10% SDS-PAGE under non-reducing conditions.
|Tested species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Highly purified protective antigen of Bacillus anthracis.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21675 detects Bacillus anthracis protective antigen in bacteria samples.
MA1-21675 has been successfully used in ELISA and Western Blot procedures.
The MA1-21675 immunogen is highly purified protective antigen of Bacillus anthracis.
Protective antigen (PA) is one of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax. Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these is toxic by itself. PA+LF forms the lethal toxin (LeTx); PA+EF forms the edema toxin (EdTx).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.