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Product Details
NE135-PAB
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Bacillus cereus Phospholipase C
Conjugate
Form
Lyophilized
Concentration
2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.5% dextran
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Target Information
Phospholipase C (PLC) is a class of membrane-associated enzymes that cleave phospholipids just before the phosphate group by hydrolizing a glycerophospholipid at the phosphodiester bond between the glycerol backbone and the phosphate group. All known eukaryotic PLCs utilize only phosphoinositides as substrates. Upon its activation, PLC hydrolyzes PIP2 to modulate numerous PIP2-dependent cellular processes. The two PIP2 cleavage products, inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol (DAG), are critical second messengers to mediate calcium signaling in animal cells. Thirteen different mammalian PLCs have been identified, grouped into six subfamilies based on their primary structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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