Reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Pyruvate oxidase (Pox) is an enzyme in the oxidoreductase family. Pyruvate Oxidase consists of four subunits with identical molecular weights. Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2, with cofactors flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium. Pyruvate oxidase is activated by the thiamine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.