|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, rat bassoon fragment (residues 738-1035) expressed as a fusion protein in E.coli.|
|Storage buffer||PBS with 0.1mM PMSF, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||0.5-5 µg/ml|
|Immunohistochemistry (IHC)||2 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20689 detects Bassoon in mouse and rat samples.
MA1-20689 has been successfully used in immunocytochemistry, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects a ~400 kDa band represeneting the Bassoon protein. Additional bands between 97-400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon.
The MA1-20689 immunogen is a recombinant, rat bassoon fragment (residues 738-1035) expressed as a fusion protein in E.coli.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Bassoon is a ~420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.