Immunohistochemistry analysis of Bax showing staining in the cytoplasm of paraffin-embedded mouse lymph node tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Bax Monoclonal antibody (336400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Bacteria, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a sequence at the N-terminal of the human protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
Members of the Bcl-2 Family (including Bcl-2, Bcl-XLand Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of Bax and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cardiolipin or MTCH2 can serve as tBID receptors during apoptosis.
33-6400 was used in immunocytochemistry to research tBID receptors served by MTCH2 or Cardiolipin during apoptosis
|Raemy E,Montessuit S,Pierredon S,van Kampen AH,Vaz FM,Martinou JC||Cell death and differentiation (23:1165)||2016|
p70S6 kinase is a target of the novel proteasome inhibitor 3,3'-diamino-4'-methoxyflavone during apoptosis in human myeloid tumor cells.
33-6400 was used in western blot to explore the effects and mechanisms of the novel flavone 3,3'-diamino-4'-methoxyflavone on acute myeloid leukemia dysfunction.
|Piedfer M,Bouchet S,Tang R,Billard C,Dauzonne D,Bauvois B||Biochimica et biophysica acta (1833:1316)||2013|
BCL-2 family proteins in peripheral T-cell lymphomas: correlation with tumour apoptosis and proliferation.
33-6400 was used in immunohistochemistry to investigate anti-apoptotic proteins in patients with peripheral T-cell lymphomas.
|Rassidakis GZ,Jones D,Lai R,Ramalingam P,Sarris AH,McDonnell TJ,Medeiros LJ||The Journal of pathology (200:240)||2003|
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
33-6400 was used in immunohistochemistry to study the role of various bcl-2 family molecules in the regulation of apoptosis and the progression of urothelial cancer.
|Korkolopoulou P,Lazaris ACh,Konstantinidou AE,Kavantzas N,Patsouris E,Christodoulou P,Thomas-Tsagli E,Davaris P||European urology (41:274)||2002|
Lysophosphatidic acid and sphingosine 1-phosphate protection of T cells from apoptosis in association with suppression of Bax.
33-6400 was used in western blot to report that LPA and S1P-mediated protection from apoptosis involves Edg GPCRs.
|Goetzl EJ,Kong Y,Mei B||Journal of immunology (Baltimore, Md. : 1950) (162:2049)||1999|
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
33-6400 was used in immunohistochemistry - paraffin section to identify diagnostic and prognostic markers for glioblastoma
|Jung Y,Joo KM,Seong DH,Choi YL,Kong DS,Kim Y,Kim MH,Jin J,Suh YL,Seol HJ,Shin CS,Lee JI,Kim JH,Song SY,Nam DH||International journal of oncology (40:1122)||2012|
Extent, relationship and prognostic significance of apoptosis and cell proliferation in synovial sarcoma.
33-6400 was used in immunohistochemistry - paraffin section to examine apoptosis and cell proliferation in synovial sarcoma
|Sun B,Sun Y,Wang J,Zhao X,Wang X,Hao X||European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) (15:258)||2006|
Cellular destruction following transmyocardial laser revascularization (TMR).
33-6400 was used in immunohistochemistry - paraffin section to observe the structural characteristics of cell death in the myocardium following transmyocardial revascularization
|Cherian SM,Bobryshev YV,Tran D,Sivaraman A,Lord RS,Cherian KM||Journal of molecular histology (36:275)||2005|
Expression of Bcl-2 family members and presence of Epstein-Barr virus in the regulation of cell growth and death in classical Hodgkin's lymphoma.
33-6400 was used in immunohistochemistry - paraffin section to assess the expression of the Bcl-2 family of proteins in classical Hodgkin's lymphoma and correlate their expression with proliferation, apoptosis, and the presence of Epstein-Barr virus
|Kim LH,Nadarajah VS,Peh SC,Poppema S||Histopathology (44:257)||2004|
Aminopeptidase-N/CD13 is a potential proapoptotic target in human myeloid tumor cells.
33-6400 was used in western blot to study the contribution of APN/CD13 to the growth and survival of acute myeloid leukemia cells in vitro.
|Piedfer M,Dauzonne D,Tang R,N'Guyen J,Billard C,Bauvois B||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:2831)||2011|
|Human||Not Cited||Glucocorticoids inhibit apoptosis during fibrosarcoma development by transcriptionally activating Bcl-xL.||Gascoyne DM,Kypta RM,Vivanco Md||The Journal of biological chemistry (278:18022)||2003|
|Human||Not Cited||The novel triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) potently enhances apoptosis induced by tumor necrosis factor in human leukemia cells.||Stadheim TA,Suh N,Ganju N,Sporn MB,Eastman A||The Journal of biological chemistry (277:16448)||2002|
Pilomatrix carcinoma with lymph node metastases.
33-6400 was used in immunohistochemistry to report on a case of pilomatrix carcinoma with lymph node metastases
|Bassarova A,Nesland JM,Sedloev T,Danielsen H,Christova S||Journal of cutaneous pathology (31:330)||2004|
|Human||Not Cited||Repeated intravesical instillations of an adenoviral vector in patients with locally advanced bladder cancer: a phase I study of p53 gene therapy.||Pagliaro LC,Keyhani A,Williams D,Woods D,Liu B,Perrotte P,Slaton JW,Merritt JA,Grossman HB,Dinney CP||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (21:2247)||2003|
|Bacteria||Not Cited||BAX expression in Hodgkin and Reed-Sternberg cells of Hodgkin's disease: correlation with clinical outcome.||Rassidakis GZ,Medeiros LJ,McDonnell TJ,Viviani S,Bonfante V,Nadali G,Vassilakopoulos TP,Giardini R,Chilosi M,Kittas C,Gianni AM,Bonadonna G,Pizzolo G,Pangalis GA,Cabanillas F,Sarris AH||Clinical cancer research : an official journal of the American Association for Cancer Research (8:488)||2002|