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Immunohistochemistry analysis of Bax showing staining in the cytoplasm of paraffin-embedded mouse lymph node tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Bax Monoclonal antibody (336400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Primate|
|Published species reactivity||Eubacteria, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a sequence at the N-terminal of the human protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Members of the Bcl-2 Family (including Bcl-2, Bcl-XLand Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of Bax and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aminopeptidase-N/CD13 is a potential proapoptotic target in human myeloid tumor cells.
33-6400 was used in western blot to study the contribution of APN/CD13 to the growth and survival of acute myeloid leukemia cells in vitro.
|Piedfer M,Dauzonne D,Tang R,N'Guyen J,Billard C,Bauvois B||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:2831)||2011|
|Human||Not Cited||Glucocorticoids inhibit apoptosis during fibrosarcoma development by transcriptionally activating Bcl-xL.||Gascoyne DM,Kypta RM,Vivanco Md||The Journal of biological chemistry (278:18022)||2003|
|Human||Not Cited||The novel triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) potently enhances apoptosis induced by tumor necrosis factor in human leukemia cells.||Stadheim TA,Suh N,Ganju N,Sporn MB,Eastman A||The Journal of biological chemistry (277:16448)||2002|
|Human||Not Cited||Repeated intravesical instillations of an adenoviral vector in patients with locally advanced bladder cancer: a phase I study of p53 gene therapy.||Pagliaro LC,Keyhani A,Williams D,Woods D,Liu B,Perrotte P,Slaton JW,Merritt JA,Grossman HB,Dinney CP||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (21:2247)||2003|
|Eubacteria||Not Cited||BAX expression in Hodgkin and Reed-Sternberg cells of Hodgkin's disease: correlation with clinical outcome.||Rassidakis GZ,Medeiros LJ,McDonnell TJ,Viviani S,Bonfante V,Nadali G,Vassilakopoulos TP,Giardini R,Chilosi M,Kittas C,Gianni AM,Bonadonna G,Pizzolo G,Pangalis GA,Cabanillas F,Sarris AH||Clinical cancer research : an official journal of the American Association for Cancer Research (8:488)||2002|
apoptosis regulator BAX; BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; bcl-2-like protein 4; BCL2 associated X protein; BCL2-associated X protein omega; Bcl2-L-4; BCL2L4