Immunohistochemistry analysis of Bax showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Bax Monoclonal antibody (336600) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a sequence at the N-terminal of the protein that is common to mouse and rat.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Members of the Bcl-2 Family (including Bcl-2, Bcl-XLand Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of Bax and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical expression of apoptosis regulating proteins and sex hormone receptors in meningiomas.
33-6600 was used in immunohistochemistry - paraffin section to define the relationship between progesterone receptor and Bcl-2 in meningiomas
|Takei H,Buckleair LW,Powell SZ||Neuropathology : official journal of the Japanese Society of Neuropathology (28:62)||2008|
|Not Applicable||Not Cited||
Hypoxia/reoxygenation induces apoptosis through a ROS-mediated caspase-8/Bid/Bax pathway in human lymphocytes.
33-6600 was used in western blot to elucidate the mechanism of hypoxia/reoxygenation-induced apoptosis in human cultured lymphocytes
|Kim BM,Chung HW||Biochemical and biophysical research communications (363:745)||2007|
|Rat||Not Cited||Regulation of necrosis of H9c2 myogenic cells upon transient energy deprivation. Rapid deenergization of mitochondria precedes necrosis and is controlled by reactive oxygen species, stress kinase JNK, HSP72 and ARC.||Yaglom JA,Ekhterae D,Gabai VL,Sherman MY||The Journal of biological chemistry (278:50483)||2003|
Immunohistochemical study of p53-associated proteins in rat brain following lithium-pilocarpine status epilepticus.
33-6600 was used in immunohistochemistry to identify proteins induced by lithium-pilocarpine-mediated status epilepticus
|Tan Z,Sankar R,Tu W,Shin D,Liu H,Wasterlain CG,Schreiber SS||Brain research (929:129)||2002|