Immunofluorescent analysis of Bax (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Bax monoclonal antibody (Product # MA5-14003) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A Synthetic peptide, aa 12-24 (Cys-GPTSSEQIMKTGA), of human Bax protein. This amino acid sequence is shared by human, mouse and rat Bax protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Western Blot (WB)||See 8 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
MA5-14003 targets Bax in Western blot, FACS, immunohistochemistry (paraffin) and ICC/IF applications and shows reactivity with Human, mouse, and Rat samples. This antibody is not recommended for Jurkat cells in Western blot applications.
The MA5-14003 immunogen is a Synthetic peptide, aa 12-24 (Cys-GPTSSEQIMKTGA), of human Bax protein. This amino acid sequence is shared by human, mouse and rat Bax protein.
Bcl-2, Bax, and Bcl-X are members of a family of proteins that regulate apoptosis. In contrast to Bcl-2alpha and Bcl-XL, overexpression of bax protein accelerates the programmed cell death (apoptosis).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Apoptosis induced by Ginkgo biloba (EGb761) in melanoma cells is Mcl-1-dependent.
MA5-14003 was used in flow cytometry to study how Ginkgo biloba extract induces apoptosis in human melanoma cells
|Wang Y,Lv J,Cheng Y,Du J,Chen D,Li C,Zhang J||PloS one (10:null)||2015|
Neuroprotective effect of resveratrol in diabetic cerebral ischemic-reperfused rats through regulation of inflammatory and apoptotic events.
MA5-14003 was used in immunohistochemistry - paraffin section to discuss the importance of diabetes in Egypt
|Mohamed HE,El-Swefy SE,Hasan RA,Hasan AA||Diabetology and metabolic syndrome (6:null)||2014|
Immunohistochemical expression of apoptosis regulating proteins and sex hormone receptors in meningiomas.
MA5-14003 was used in immunohistochemistry - paraffin section to define the relationship between progesterone receptor and Bcl-2 in meningiomas
|Takei H,Buckleair LW,Powell SZ||Neuropathology : official journal of the Japanese Society of Neuropathology (28:62)||2008|
Phosphorylated nerve growth factor-induced clone B (NGFI-B) translocates from the nucleus to mitochondria of stressed rat cardiomyocytes and induces apoptosis.
MA5-14003 was used in western blot to study the effects of stress on the nuclear to mitochondrial translocation of NGFI-B and its role in the induction of cardiomyocyte apoptosis
|Xinxing W,Hong F,Rui Z,Yun Z,Jingbo G,Lingjia Q||Stress (Amsterdam, Netherlands) (15:545)||2012|
Protective effects of trimetazidine on bone marrow mesenchymal stem cells viability in an ex vivo model of hypoxia and in vivo model of locally myocardial ischemia.
MA5-14003 was used in western blot to study the effect of trimetazidine on the survival of bone marrow mesenchymal stem cells in a model of hypoxia and following transplantation into rat heart
|Xu H,Zhu G,Tian Y||Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (32:36)||2012|
Prenatal stress induces long-term effects in cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats.
MA5-14003 was used in western blot to study the long-term effects of sub-chronic prenatal stress on cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats
|Baquedano E,García-Cáceres C,Diz-Chaves Y,Lagunas N,Calmarza-Font I,Azcoitia I,Garcia-Segura LM,Argente J,Chowen JA,Frago LM||PloS one (6:null)||2011|
Combined treatment with the Cox-2 inhibitor niflumic acid and PPAR¿ ligand ciglitazone induces ER stress/caspase-8-mediated apoptosis in human lung cancer cells.
MA5-14003 was used in western blot to evaluate a combination treatment of niflumic acid and ciglitazone in human lung cancer cells
|Kim BM,Maeng K,Lee KH,Hong SH||Cancer letters (300:134)||2011|
Cell cycle arrest and cytochrome c-mediated apoptotic induction by MCS-5A is associated with up-regulation of p16(INK4a) in HL-60 cells.
MA5-14003 was used in western blot to investigate the effect of cyclin-dependent kinase inhibitor on the cell cycle and apoptosis in HL-60 cells and the underlying mechanism
|Choi BY,Lee CH||Bioorganic and medicinal chemistry letters (20:3880)||2010|
Desferrioxamine (DFX) induces apoptosis through the p38-caspase8-Bid-Bax pathway in PHA-stimulated human lymphocytes.
MA5-14003 was used in western blot to investigate the effect of desferrioxamine on apoptosis and its mechanism
|Kim BM,Chung HW||Toxicology and applied pharmacology (228:24)||2008|
|Not Applicable||Not Cited||
Hypoxia/reoxygenation induces apoptosis through a ROS-mediated caspase-8/Bid/Bax pathway in human lymphocytes.
MA5-14003 was used in western blot to elucidate the mechanism of hypoxia/reoxygenation-induced apoptosis in human cultured lymphocytes
|Kim BM,Chung HW||Biochemical and biophysical research communications (363:745)||2007|
Influence of glutathione on the induction of chromosome aberrations, delay in cell cycle kinetics and cell cycle regulator proteins in irradiated mouse bone marrow cells.
MA5-14003 was used in western blot to investigate the effect of glutathione on chromosome aberrations, cell cycle kinetics, and cell cycle regulator proteins
|Ray S,Chatterjee A||International journal of radiation biology (83:347)||2007|
Sequential caspase-2 and caspase-8 activation is essential for saikosaponin a-induced apoptosis of human colon carcinoma cell lines.
MA5-14003 was used in immunocytochemistry to investigate the role of caspase activation in the apoptotic effect of saikosaponin in colon carcinoma cell lines
|Kim BM,Hong SH||Apoptosis : an international journal on programmed cell death (16:184)||2011|
The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.
MA5-14003 was used in immunocytochemistry to investigate the relationship and interaction between Bax and Bcl-xL
|Parikh N,Koshy C,Dhayabaran V,Perumalsamy LR,Sowdhamini R,Sarin A||BMC cell biology (8:null)||2007|
Iptakalim protects PC12 cell against H2O2-induced oxidative injury via opening mitochondrial ATP-sensitive potassium channel.
MA5-14003 was used in immunocytochemistry to study the protection of PC12 cells against H2O2-induced oxidative injury by iptakalim-mediated opening of mitochondrial ATP-sensitive potassium channels
|Chai Y,Niu L,Sun XL,Ding JH,Hu G||Biochemical and biophysical research communications (350:307)||2006|
(+/-)-huprine Y, (-)-huperzine A and tacrine do not show neuroprotective properties in an apoptotic model of neuronal cytoskeletal alteration.
MA5-14003 was used in immunocytochemistry to study the effect of acetylcholinesterase inhibitors in colchicine-induced apoptosis in cerebellar granule neurons
|Jordá EG,Verdaguer E,Jiménez A,Canudas AM,Rimbau V,Camps P,Muñoz-Torrero D,Camins A,Pallàs M||Journal of Alzheimer's disease : JAD (6:577)||2004|
Heme oxygenase-1 prevents hyperthyroidism induced hepatic damage via an antioxidant and antiapoptotic pathway.
MA5-14003 was used in immunohistochemistry to investigate the protective effect of heme oxygenase-1 against hyperthyroidism and its mechanism
|Giri¿ M,Erbil Y,Depboylu B,Mete O,Türko¿lu U,Abbaso¿lu SD,Uysal M||The Journal of surgical research (164:266)||2010|
Notch-activated signaling cascade interacts with mitochondrial remodeling proteins to regulate cell survival.
MA5-14003 was used in immunohistochemistry to investigate the interaction between notch signalling and mitochondrial remodeling proteins and its effect on cell survival
|Perumalsamy LR,Nagala M,Sarin A||Proceedings of the National Academy of Sciences of the United States of America (107:6882)||2010|
N,N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells.
MA5-14003 was used in immunoprecipitation to investigate the mechanism for apoptosis induced by N,N-dimethyl phytosphingosine in human leukemia cells
|Kim BM,Choi YJ,Han Y,Yun YS,Hong SH||Toxicology and applied pharmacology (239:87)||2009|