This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details
MA1-26233 detects Bcl2 from human samples.
MA1-26233 has been successfully used in immunoprecipitation, Immunohistochemistry, and Western blot procedures. Positive control of Tonsil or human lung cell line suggested. This antibody works well with B5 or Bouin's fixed tissues. Formalin fixed tissues may give inconsistent results.
The MA1-26233 immunogen is recombinant, peptide sequence corresponding to residues 41-54 of Bcl-2 oncoprotein.
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; protein phosphatase 1, regulatory subunit 50
Gene Aliases: Bcl-2; BCL2; PPP1R50
UniProt ID: (Human) Q16197
Entrez Gene ID: (Human) 596
Molecular Function: signaling molecule