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Invitrogen

Bcl-xL Recombinant Rabbit Monoclonal Antibody (016)

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Bcl-xL Recombinant Rabbit Monoclonal Antibody (016)

Product Details

MA5-29049

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

5 µg
-

ELISA (ELISA)

1:25,000-1:50,000
-

Immunoprecipitation (IP)

0.2-1 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

016

Immunogen

Recombinant Human BCL2L1/Bcl-XL Protein (Met1-Arg212)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784983

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human BCL2L1/Bcl-XL.

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptosis regulator Bcl-X; bcl-xL; bcl-xS; BCLX; BCLXL; BCLXS; DKFZp781P2092; OTTHUMP00000030550; OTTHUMP00000030552; OTTHUMP00000030555; OTTHUMP00000030556; OTTHUMP00000030557; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3; unnamed protein product

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Gene Aliases: Bcl-X; BCL-XL/S; BCL2L; BCLX; PPP1R52

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UniProt ID: (Human) Q07817

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Entrez Gene ID: (Human) 598

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Function(s)
protein binding channel activity protein kinase binding identical protein binding BH3 domain binding
Process(es)
release of cytochrome c from mitochondria response to stress intrinsic apoptotic signaling pathway in response to DNA damage apoptotic mitochondrial changes negative regulation of autophagy programmed cell death regulation of cytokinesis response to cytokine regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process regulation of mitochondrial membrane permeability defense response to virus response to other organism regulation of mitochondrial membrane potential transmembrane transport negative regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway in absence of ligand negative regulation of execution phase of apoptosis negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway regulation of intracellular signal transduction negative regulation of protein localization to plasma membrane negative regulation of anoikis regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway
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