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Western blot analysis of Beta-Actin was performed by loading 20ug of THP-1 whole cell lysate per well onto a SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The membrane was probed with a Beta-Actin monoclonal antibody (Product # MA5-15739) at a dilution of 1:5000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse, Guinea pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Beta-actin N-terminal peptide|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1-2 ug/test|
|Western Blot (WB)||1:1000 - 1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 10 publications below|
MA5-15739 detects beta actin from human, mouse, rat, rabbit, and chicken samples.
MA5-15739 has been successfully used in Western blot, FACS, and ELISA.
MA5-15739 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amylin deposition in the brain: A second amyloid in Alzheimer disease?
MA5-15739-1MG was used in western blot to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease
|Jackson K,Barisone GA,Diaz E,Jin LW,DeCarli C,Despa F||Annals of neurology (74:517)||2013|
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
MA5-15739-1MG was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats
|Castro B,Sánchez P,Torres JM,Ortega E||PloS one (8:null)||2013|
Catecholaminergic-induced arrhythmias in failing cardiomyocytes associated with human HRCS96A variant overexpression.
MA5-15739 was used in western blot to investigate the relationship between histidine-rich calcium binding protein HRC S96A variant overexpression and human arrhythmias
|Han P,Cai W,Wang Y,Lam CK,Arvanitis DA,Singh VP,Chen S,Zhang H,Zhang R,Cheng H,Kranias EG||American journal of physiology. Heart and circulatory physiology (301:H1588)||2011|
Deletion of the p85alpha regulatory subunit of phosphoinositide 3-kinase in cone photoreceptor cells results in cone photoreceptor degeneration.
MA5-15739 was used in western blot to investigate the role of phosphoinositide 3-kinase p85alpha subunit for the development and survival of cone photoreceptors
|Ivanovic I,Anderson RE,Le YZ,Fliesler SJ,Sherry DM,Rajala RV||Investigative ophthalmology & visual science (52:3775)||2011|
An extract of Artemisia dracunculus L. enhances insulin receptor signaling and modulates gene expression in skeletal muscle in KK-A(y) mice.
MA5-15739 was used in western blot to investigate the effect of Artemisia dracunculus L. extract PMI 5011 on insulin sensitivity and insulin receptor signaling
|Wang ZQ,Ribnicky D,Zhang XH,Zuberi A,Raskin I,Yu Y,Cefalu WT||The Journal of nutritional biochemistry (22:71)||2011|
Androgens stimulate telomerase expression, activity and phosphorylation in ovarian adenocarcinoma cells.
MA5-15739 was used in western blot to investigate the effect of androgens on telomerase in ovarian adenocarcinoma cells
|Nourbakhsh M,Golestani A,Zahrai M,Modarressi MH,Malekpour Z,Karami-Tehrani F||Molecular and cellular endocrinology (330:10)||2010|
Hypermethylation of HLA class I gene is associated with HLA class I down-regulation in human gastric cancer.
MA5-15739 was used in western blot to investigate the effect of methylation status on HLA class I expression in cancers
|Ye Q,Shen Y,Wang X,Yang J,Miao F,Shen C,Zhang J||Tissue antigens (75:30)||2010|
Homologous recombination but not nucleotide excision repair plays a pivotal role in tolerance of DNA-protein cross-links in mammalian cells.
MA5-15739 was used in western blot to study the role of hmologous recombination and nucleotide excision repair in tolerance of DNA-protein cross-links in mammalian cells
|Nakano T,Katafuchi A,Matsubara M,Terato H,Tsuboi T,Masuda T,Tatsumoto T,Pack SP,Makino K,Croteau DL,Van Houten B,Iijima K,Tauchi H,Ide H||The Journal of biological chemistry (284:27065)||2009|
Induction of ERBB2 nuclear transport after radiation in breast cancer cells.
MA5-15739 was used in western blot to investigate the nuclear transport of ERBB2 in breast cancer cells after radiation
|Luo B,Yu S,Zhuang L,Xia S,Zhao Z,Rong L||Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (29:350)||2009|
SUMO-specific protease 2 is essential for modulating p53-Mdm2 in development of trophoblast stem cell niches and lineages.
MA5-15739 was used in western blot to investigate the influence of SUMO-specific protease 2 on trophoblast lineage development
|Chiu SY,Asai N,Costantini F,Hsu W||PLoS biology (6:null)||2008|
actin, beta; actin, cytoplasmic 1; Atcb; beta cytoskeletal actin; beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1
ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19