Western blot analysis of Beta-Actin was performed by loading 20ug of THP-1 whole cell lysate per well onto a SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The membrane was probed with a Beta-Actin monoclonal antibody (Product # MA5-15739) at a dilution of 1:5000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable, Guinea pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Beta-actin N-terminal peptide|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1-2 ug/test|
|Western Blot (WB)||1:1000 - 1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 12 publications below|
MA5-15739 detects beta actin from human, mouse, rat, rabbit, and chicken samples.
MA5-15739 has been successfully used in Western blot, FACS, and ELISA.
MA5-15739 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma.
MA5-15739-1MG was used in western blot to analyze prevention of metastasis in murine mammary carcinoma by targeting serglycin
|Roy A,Femel J,Huijbers EJ,Spillmann D,Larsson E,Ringvall M,Olsson AK,Åbrink M||PloS one (11:null)||2016|
NETosing Neutrophils Activate Complement Both on Their Own NETs and Bacteria via Alternative and Non-alternative Pathways.
MA5-15739-1MG was used in western blot to compare activation of complement both on their own NETs and bacteria via non-alternative and alternative pathways by NETosing neutrophils
|Yuen J,Pluthero FG,Douda DN,Riedl M,Cherry A,Ulanova M,Kahr WH,Palaniyar N,Licht C||Frontiers in immunology (7:null)||2016|
Amylin deposition in the brain: A second amyloid in Alzheimer disease?
MA5-15739-1MG was used in western blot to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease
|Jackson K,Barisone GA,Diaz E,Jin LW,DeCarli C,Despa F||Annals of neurology (74:517)||2013|
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
MA5-15739-1MG was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats
|Castro B,Sánchez P,Torres JM,Ortega E||PloS one (8:null)||2013|
|Guinea pig||Not Cited||
NCX is an important determinant for premature ventricular activity in a drug-induced model of Andersen-Tawil syndrome.
MA5-15739 was used in western blot to investigate the involvement of NCX in arrhythmias in a model of Andersen-Tawil syndrome
|Radwa¿ski PB,Poelzing S||Cardiovascular research (92:57)||2011|
Deletion of the p85alpha regulatory subunit of phosphoinositide 3-kinase in cone photoreceptor cells results in cone photoreceptor degeneration.
MA5-15739 was used in western blot to investigate the role of phosphoinositide 3-kinase p85alpha subunit for the development and survival of cone photoreceptors
|Ivanovic I,Anderson RE,Le YZ,Fliesler SJ,Sherry DM,Rajala RV||Investigative ophthalmology and visual science (52:3775)||2011|
An extract of Artemisia dracunculus L. enhances insulin receptor signaling and modulates gene expression in skeletal muscle in KK-A(y) mice.
MA5-15739 was used in western blot to investigate the effect of Artemisia dracunculus L. extract PMI 5011 on insulin sensitivity and insulin receptor signaling
|Wang ZQ,Ribnicky D,Zhang XH,Zuberi A,Raskin I,Yu Y,Cefalu WT||The Journal of nutritional biochemistry (22:71)||2011|
Androgens stimulate telomerase expression, activity and phosphorylation in ovarian adenocarcinoma cells.
MA5-15739 was used in western blot to investigate the effect of androgens on telomerase in ovarian adenocarcinoma cells
|Nourbakhsh M,Golestani A,Zahrai M,Modarressi MH,Malekpour Z,Karami-Tehrani F||Molecular and cellular endocrinology (330:10)||2010|
Hypermethylation of HLA class I gene is associated with HLA class I down-regulation in human gastric cancer.
MA5-15739 was used in western blot to investigate the effect of methylation status on HLA class I expression in cancers
|Ye Q,Shen Y,Wang X,Yang J,Miao F,Shen C,Zhang J||Tissue antigens (75:30)||2010|
Homologous recombination but not nucleotide excision repair plays a pivotal role in tolerance of DNA-protein cross-links in mammalian cells.
MA5-15739 was used in western blot to study the role of hmologous recombination and nucleotide excision repair in tolerance of DNA-protein cross-links in mammalian cells
|Nakano T,Katafuchi A,Matsubara M,Terato H,Tsuboi T,Masuda T,Tatsumoto T,Pack SP,Makino K,Croteau DL,Van Houten B,Iijima K,Tauchi H,Ide H||The Journal of biological chemistry (284:27065)||2009|
Induction of ERBB2 nuclear transport after radiation in breast cancer cells.
MA5-15739 was used in western blot to investigate the nuclear transport of ERBB2 in breast cancer cells after radiation
|Luo B,Yu S,Zhuang L,Xia S,Zhao Z,Rong L||Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (29:350)||2009|
SUMO-specific protease 2 is essential for modulating p53-Mdm2 in development of trophoblast stem cell niches and lineages.
MA5-15739 was used in western blot to investigate the influence of SUMO-specific protease 2 on trophoblast lineage development
|Chiu SY,Asai N,Costantini F,Hsu W||PLoS biology (6:null)||2008|