Western blot analysis of Beta-Actin was performed by loading 30 µg of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated Beta-Actin monoclonal antibody (Product # MA5-15739-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).
|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Beta-actin N-terminal peptide.|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA5-15739-BTIN has been successfully used for Western blot and FACS applications.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amylin deposition in the brain: A second amyloid in Alzheimer disease?
MA5-15739-BTIN was used in western blot to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease
|Jackson K,Barisone GA,Diaz E,Jin LW,DeCarli C,Despa F||Annals of neurology (74:517)||2013|
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
MA5-15739-BTIN was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats
|Castro B,Sánchez P,Torres JM,Ortega E||PloS one (8:null)||2013|