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Immunofluorescent analysis of beta-actin (red) in HeLa cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, blocked with 0.3% BSA in PBS for 15 minutes, and probed with a DyLight 800-conjugated beta-actin monoclonal antibody (Product # MA5-15739-D800) at a dilution of 1:100 overnight at 4°C. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on an EVOS® FL Auto Cell Imaging Station at 20X magnification.
|Tested species reactivity||Chicken , Human , Mouse , Rabbit , Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Beta-actin N-terminal peptide.|
|Conjugate||DyLight 800 4X PEG|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15739-D800 has been successfully used for Western blotting and immunofluorescence. DyLight 800 4X PEG has an excitation/emission of 783/797 nm. For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ACTB; actin, beta; actin, cytoplasmic 1; Atcb; beta cytoskeletal actin; beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1
ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19