Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (Product # MA5-16308) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Avian, Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||KLH conjugated peptide of the N-terminus of Beta-Tubulin.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:2000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 22 publications below|
MA5-16308 detects beta-tubulin in human, monkey, mouse, rat, rabbit and chicken samples with ELISA, FACS, IHC (P), Western blot and immunofluorescence applications.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth.
MA5-16308 was used in western blot to demonstrate the key role of essential amino acid transport in the control of mTORC1 and tumor growth caused by genetic disruption of the multifunctional CD98/LAT1 complex
|Cormerais Y,Giuliano S,LeFloch R,Front B,Durivault J,Tambutté E,Massard PA,de la Ballina LR,Endou H,Wempe MF,Palacin M,Parks SK,Pouyssegur J||Cancer research (76:4481)||2016|
The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.
MA5-16308 was used in western blot to investigate the requirement for EIPR-1 and the EARP complex for cargo sorting to dense-core vesicles
|Topalidou I,Cattin-Ortolá J,Pappas AL,Cooper K,Merrihew GE,MacCoss MJ,Ailion M||PLoS genetics (12:null)||2016|
Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
MA5-16308 was used in western blot to study the differential effects of a high fat diet and caloric restriction on murne hypothalamic CNTF signaling
|Severi I,Perugini J,Mondini E,Smorlesi A,Frontini A,Cinti S,Giordano A||Frontiers in neuroscience (7:null)||2014|
The tyrosine phosphatase PTPN14 is a negative regulator of YAP activity.
MA5-16308 was used in western blot to study the mechanism by which PTPN14 modulates YAP activity and the significance for oncogenesis
|Michaloglou C,Lehmann W,Martin T,Delaunay C,Hueber A,Barys L,Niu H,Billy E,Wartmann M,Ito M,Wilson CJ,Digan ME,Bauer A,Voshol H,Christofori G,Sellers WR,Hofmann F,Schmelzle T||PloS one (8:null)||2013|
Carvedilol treatment after myocardial infarct decreases cardiomyocytic apoptosis in the peri-infarct zone during cardioplegia-induced cardiac arrest.
MA5-16308 was used in western blot to study the protective effects of carvedilol against cardiomyocyte apoptosis in a model of hypoxia-reperfusion injury
|Yeh CH,Chen TP,Wang YC,Lin YM,Fang SW||Shock (Augusta, Ga.) (39:343)||2013|
Inositol 1,4,5-trisphosphate receptor regulates replication, differentiation, infectivity and virulence of the parasitic protist Trypanosoma cruzi.
MA5-16308 was used in western blot to identify and characterize novel Trypanosoma cruzi IP3 receptors and their role in virulence and infectivity
|Hashimoto M,Enomoto M,Morales J,Kurebayashi N,Sakurai T,Hashimoto T,Nara T,Mikoshiba K||Molecular microbiology (87:1133)||2013|
STAT1 activation by venous malformations mutant Tie2-R849W antagonizes VEGF-A-mediated angiogenic response partly via reduced bFGF production.
MA5-16308 was used in western blot to study the role of STAT1 in the molecular mechanism of a venous malformation-associated Tie-2 mutation
|Huang YH,Wu MP,Pan SC,Su WC,Chen YW,Wu LW||Angiogenesis (16:207)||2013|
MicroRNA-27a regulates cardiomyocytic apoptosis during cardioplegia-induced cardiac arrest by targeting interleukin 10-related pathways.
MA5-16308 was used in western blot to study the involvement of microRNA-27a in the apoptosis of cardiomyocytes in response to cardioplegic cardiac arrest during cardiopulmonary bypass
|Yeh CH,Chen TP,Wang YC,Lin YM,Fang SW||Shock (Augusta, Ga.) (38:607)||2012|
Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes.
MA5-16308 was used in western blot to study the role of EGFR and CDC25B in the mechanism by which indirubin inhibits epidermal keratinicyte proliferation
|Hsieh WL,Lin YK,Tsai CN,Wang TM,Chen TY,Pang JH||Journal of dermatological science (67:140)||2012|
Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria.
MA5-16308 was used in western blot to study the use of mutant fch/fch mice as a model of Mallory-Denk body formation
|Singla A,Moons DS,Snider NT,Wagenmaker ER,Jayasundera VB,Omary MB||Hepatology (Baltimore, Md.) (56:322)||2012|
DNA methyltransferase 1, cytosine methylation, and cytosine hydroxymethylation in mammalian mitochondria.
MA5-16308 was used in western blot to study the role of mitochondrial DNA methyltransferase-1 in the cytosine methylation of mitochondrial DNA
|Shock LS,Thakkar PV,Peterson EJ,Moran RG,Taylor SM||Proceedings of the National Academy of Sciences of the United States of America (108:3630)||2011|
Interdependence of platelet-derived growth factor and estrogen-signaling pathways in inducing neonatal rat testicular gonocytes proliferation.
MA5-16308 was used in western blot to study the interdependence of PDGF and estrogen signaling and their role in gonocyte development
|Thuillier R,Mazer M,Manku G,Boisvert A,Wang Y,Culty M||Biology of reproduction (82:825)||2010|
Grape seed extract induces cell cycle arrest and apoptosis in human colon carcinoma cells.
MA5-16308 was used in western blot to study the effects on human colon carcinoma cell growth and apoptosis of grape seed extract
|Kaur M,Mandair R,Agarwal R,Agarwal C||Nutrition and cancer (60 Suppl 1:2)||2008|
Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1.
MA5-16308 was used in western blot to study the role of EWS-FLI1 in neural differentiation and tumorigenesis
|Yang Y,Zhang L,Wei Y,Wang H,Fukuma M,Xu H,Xiong W,Zheng J||Journal of neuro-oncology (90:141)||2008|
Growth control of multiple myeloma cells through inhibition of glycogen synthase kinase-3.
MA5-16308 was used in western blot to study the role of GSK-3 in modulating the growth of multiple myeloma cells
|Zhou Y,Uddin S,Zimmerman T,Kang JA,Ulaszek J,Wickrema A||Leukemia and lymphoma (49:1945)||2008|
Constitutive overexpression of CDC25A in primary human mammary epithelial cells results in both defective DNA damage response and chromosomal breaks at fragile sites.
MA5-16308 was used in western blot to study the role of CDC25A phosphatase overexpression in promoting genomic instability in primary human mammary epithelial cells
|Cangi MG,Piccinin S,Pecciarini L,Talarico A,Dal Cin E,Grassi S,Grizzo A,Maestro R,Doglioni C||International journal of cancer (123:1466)||2008|
Endothelial nitric oxide synthase in bicuspid aortic valve disease.
MA5-16308 was used in western blot to study the potential role of eNOS in the pathology of bicuspid aortic valve disease
|Aicher D,Urbich C,Zeiher A,Dimmeler S,Schäfers HJ||The Annals of thoracic surgery (83:1290)||2007|
Stimulation of non-transferrin iron uptake by iron deprivation in K562 cells.
MA5-16308 was used in western blot to study the effect of iron deprivation on the uptake of non-transferrin iron in human erythroleukemia cells
|Kovar J,Neubauerova J,Cimburova M,Truksa J,Balusikova K,Horak J||Blood cells, molecules and diseases (37:95)||2006|
Erbin regulates mitogen-activated protein (MAP) kinase activation and MAP kinase-dependent interactions between Merlin and adherens junction protein complexes in Schwann cells.
MA5-16308 was used in western blot to study the interaction between Merlin and Schwann cell adherens junction proteins and the role of erbin and MAP kinases
|Rangwala R,Banine F,Borg JP,Sherman LS||The Journal of biological chemistry (280:11790)||2005|
Mechanical stretch induces podocyte hypertrophy in vitro.
MA5-16308 was used in western blot to study the potential role of p21 and p27 in the induction of podocyte hypertrophy by mechanical stretch
|Petermann AT,Pippin J,Durvasula R,Pichler R,Hiromura K,Monkawa T,Couser WG,Shankland SJ||Kidney international (67:157)||2005|
TorsinA negatively controls neurite outgrowth of SH-SY5Y human neuronal cell line.
MA5-16308 was used in western blot to study the role of torsinA in modulating neurite outgrowth in a human neuronal cell line
|Ferrari-Toninelli G,Paccioretti S,Francisconi S,Uberti D,Memo M||Brain research (1012:75)||2004|
Nicotine strongly activates dendritic cell-mediated adaptive immunity: potential role for progression of atherosclerotic lesions.
MA5-16308 was used in western blot to study the ability of nicotine to stimulate dendritic cell function and the significance for atherogenesis
|Aicher A,Heeschen C,Mohaupt M,Cooke JP,Zeiher AM,Dimmeler S||Circulation (107:604)||2003|