Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (Product # MA5-16308) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||KLH conjugated peptide of the N-terminus of Beta-Tubulin.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA5-16308-1MG detects beta-tubulin in human, monkey, mouse, rat, rabbit and chicken samples with ELISA, FACS, Western blot, IHC (P) and immunofluorescence applications.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.
MA5-16308-1MG was used in western blot to investigate the requirement for EIPR-1 and the EARP complex for cargo sorting to dense-core vesicles
|Topalidou I,Cattin-Ortolá J,Pappas AL,Cooper K,Merrihew GE,MacCoss MJ,Ailion M||PLoS genetics (12:null)||2016|
Early adaptive response of the retina to a pro-diabetogenic diet: Impairment of cone response and gene expression changes in high-fructose fed rats.
MA5-16308-1MG was used in western blot to study the adaptive response of the retina to short-term high fructose treatment
|Thierry M,Pasquis B,Buteau B,Fourgeux C,Dembele D,Leclere L,Gambert-Nicot S,Acar N,Bron AM,Creuzot-Garcher CP,Bretillon L||Experimental eye research (135:37)||2015|