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Immunofluorescent analysis of Beta-Tubulin (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 650-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-D650) at a dilution of 1:50 for at least 1 hour at room temperature. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||KLH conjugated peptide of the N-terminus of Beta-Tubulin.|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:25 - 1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16308-D650 has successfully been used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672nm.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
beta 5-tubulin; beta Ib tubulin; beta polypeptide; beta-4 tubulin; c(beta)7 tubulin; tubulin; Tubulin beta 4'; Tubulin beta chain; tubulin beta polypeptide; Tubulin beta-1 chain; Tubulin beta-5 chain; Tubulin beta-7 chain; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta class I; tubulin, beta polypeptide
2810484G07Rik; CDCBM6; CSCSC1; M(beta)1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB2A; TUBB5