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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide from near the carboxy terminus of human Bfl-1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||10 ug/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Despite the predicted molecular weight, Bfl-1 often migrates at a higher molecular weight in SDS-PAGE, presumably due to post-translational modifications. A suggested positive control is human kidney tissue lysate.
PA5-20268 can be used with blocking peptide PEP-0383.
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A1-A; ACC-1, ACC-2, BCL2L5, BFL1, GRS, HBPA1; B-cell leukemia/lymphoma 2 related protein A1a; bcl-2-like protein 5; bcl-2-related protein A1; bcl2-L-5; BCL2-related protein A1; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein; protein BFL-1
A1; ACC-1; ACC-2; ACC1; ACC2; BB218357; BCL2A1; Bcl2a1a; BCL2L5; Bfl-1; BFL1; GRS; HBPA1