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Immunohistochemical analysis of formalin-fixed, paraffin-embedded tissue sections stained for Bi-1 expression using a Bi-1 monoclonal antibody (Product # MA1-41108) at 5 ug/ml. A: normal human lung alveoli with positive lung macrophages. B: normal mouse cartilage with positive chondrocytes. Hematoxylin-eosin counterstain.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Synthetic peptides corresponding to the N-terminal, internal, and C-terminal regions of human B1.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.
BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress–causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, it is thought to involve regulation of Ca2+ handling by the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bax inhibitor 1; Bax inhibitor-1; BAXI1; BI-1; TEGT; testis enhanced gene transcript; testis-enhanced gene transcript protein; transmembrane BAX inhibitor motif-containing protein 6
5031406P05Rik; Ab1-011; Ac1-149; BAXI1; BI-1; BI1; Cc1-27; TEGT; TMBIM6