Osenses

Bif-1 Polyclonal Antibody

View all (12) Bif1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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Bif-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Bif-1 Antibody in Western Blot (WB) — WB on mouse tissue lysates. Each lysate was prepared individually and then mixed together. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:8000 incubated at 4°C overnight.

Product Details

OSE00030W-100UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:8,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide from human Bif conjugated to blue carrier protein was used as the antigen
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	whole serum
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat and mouse.

Specificity of this antibody: Bif-1.

Target Information

Bif1 gene encodes a protein similar to a protein in rodents which is induced by bone morphogenic protein 2 in vitro and may be involved in BMP2-induced transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bax-interacting factor 1; Bif-1; BIF1; endophilin b1; Endophilin-B1; protein phosphatase 1, regulatory subunit 70; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like B1 (endophilin); SH3-domain GRB2-like endophilin B1; sh3glb1 {ECO:0000312|EMBL:AAH79085.1}; testicular tissue protein Li 172

Gene Aliases: AA409932; AI314629; AU015566; Bif-1; CGI-61; dJ612B15.2; KIAA0491; mKIAA0491; PPP1R70; SH3GLB1

UniProt ID: (Human) Q9Y371, (Rat) Q6AYE2, (Mouse) Q9JK48

Entrez Gene ID: (Human) 51100, (Rat) 292156, (Mouse) 54673

Function(s)