|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified human biliverdin-IX alpha-reductase expressed in E. coli.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-019 detects biliverdin-IX alpha reductase (BLVRA) from human cells.
PA1-019 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing BLVRA from HeLa cell extract.
The PA1-019 immunogen is purified human biliverdin reductase expressed in E. coli.
Biliverdin reductase (BLVR) is a ubiquitously expressed, small cytoplasmic oxidoreductase that catalyzes the reduction of biliverdin to bilirubin. The first step of this process is catalyzed by heme oxygenase, an enzyme that converts heme to iron, carbon monoxide, and biliverdin. BLVR is unique among all enzymes to date in being dual pH/dual cofactor-dependent, as it requires NADH (pH 6.7) or NADPH (pH 8.7) as an electron donor. There are two isoforms of BLVR, biliverdin-IX alpha-reductase (BLVRA), the major component of human adult liver, and biliverdin–IX beta-reductase (BLVRB), found predominantly in fetal liver. The product of BLVR, bilirubin, is an important biological antioxidant, however it is also a neurotoxin and the cause of kernicterus. In addition to being an antioxidant, it inhibits kinase activity and the activity of enzymes such as protein kinase C and NADPH oxidase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Biliverdin reductase: a major physiologic cytoprotectant.
PA1-019 was used in western blot to study the antioxidant and cytoprotective properties of biliverdin reductase
|Baranano DE,Rao M,Ferris CD,Snyder SH||Proceedings of the National Academy of Sciences of the United States of America (99:16093)||2002|
Studies on the specificity of the tetrapyrrole substrate for human biliverdin-IXalpha reductase and biliverdin-IXbeta reductase. Structure-activity relationships define models for both active sites.
PA1-019 was used in western blot to investigate the substrate specificity of recombinant human BVR-A and BVR-B
|Cunningham O,Dunne A,Sabido P,Lightner D,Mantle TJ||The Journal of biological chemistry (275:19009)||2000|