|Flow Cytometry (FACS)||10 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||1-2 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG1|
|Immunogen||Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
For flow cytometry applications, it is recommend to permeabilize cells with 0.03% saponin.
Purity is > 95% by SDS-PAGE.
Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bcl-2-like protein 11; Bcl2 interacting mediator of cell death; bcl2-interacting mediator of cell death
Gene Aliases: 1500006F24Rik; bcl2-L-11; Bim; Bod
UniProt ID: (Mouse) O54918
Entrez Gene ID: (Mouse) 12125
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