|Tested species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG1|
|Immunogen||Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (FACS)||10 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||1-2 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For flow cytometry applications, it is recommend to permeabilize cells with 0.03% saponin.
Purity is > 95% by SDS-PAGE.
Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.