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|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Biotin conjugated to BSA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|In Situ Hybridization (ISH) (ISH)||Assay dependent|
|Western Blot (WB)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11251 targets Biotin in Western blot, immunomicroscopy, in situ hybridization and flow cytometry applications and shows reactivity with All samples.
The MA5-11251 immunogen is biotin conjugated to BSA.
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Modulating protein activity using tethered ligands with mutually exclusive binding sites.
MA5-11251 was used in ELISA to establish a method to modulate protein activity via a specific effector
|Schena A,Griss R,Johnsson K||Nature communications (6:null)||2015|
|Not Applicable||0.5 ug/ml||
A yeast display immunoprecipitation method for efficient isolation and characterization of antigens.
MA5-11251 was used in western blot to develop a novel yeast display immunoprecipitation method for antigen isolation and characterization
|Cho YK,Chen I,Wei X,Li L,Shusta EV||Journal of immunological methods (341:117)||2009|