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|Tested species reactivity||Chemical|
|Published species reactivity||Bacteria, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||1 µl/ml||
Quantitative perturbation-based analysis of gene expression predicts enhancer activity in early Drosophila embryo.
03-3700 was used in immunohistochemistry to study the prediction of enhancer activity in early drosophila embryo due to quantitative perturbation-based analysis of gene expression
|Sayal R,Dresch JM,Pushel I,Taylor BR,Arnosti DN||eLife (5:null)||2016|
|Not Applicable||3 µg/ml||
Intercellular adhesion molecule 1 promotes HIV-1 attachment but not fusion to target cells.
MA1-19021 was used in blocking or activating experiment to determine the role of ICAM-1/LFA-1 interactions in productive HIV-1 entry into lymphoid cells
|Kondo N,Melikyan GB||PloS one (7:null)||2012|
|Not Applicable||Not Cited||
Cell type-specific regulation of ITAM-mediated NF-kappaB activation by the adaptors, CARMA1 and CARD9.
03-3700 was used in blocking or activating experiment to study the impact of the caspase recruitment domain 9-Bcl10 complex on NK cell receptor signaling
|Hara H,Ishihara C,Takeuchi A,Xue L,Morris SW,Penninger JM,Yoshida H,Saito T||Journal of immunology (Baltimore, Md. : 1950) (181:918)||2008|
Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques.
03-3700 was used in immunohistochemistry - free floating to analyze false-positive results with avidin-biotin and streptavidin-biotin techniques due to endogenous biotin in rat brain
|McKay BE,Molineux ML,Turner RW||Methods in molecular biology (Clifton, N.J.) (418:111)||2008|
|Human||Not Cited||Specific patterns of electrophile adduction trigger Keap1 ubiquitination and Nrf2 activation.||Hong F,Sekhar KR,Freeman ML,Liebler DC||The Journal of biological chemistry (280:31768)||2005|
|Bacteria||Not Cited||Heme binding to the histidine-rich protein II from Plasmodium falciparum.||Schneider EL,Marletta MA||Biochemistry (44:979)||2005|
||Heme binding to the histidine-rich protein II from Plasmodium falciparum.||Schneider EL,Marletta MA||Biochemistry (44:979)||2005|