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Immunohistochemistry was performed on formalin-fixed paraffin-embedded pancreatic adenocarcinoma tissue sections. Heat-induced epitope retrieval was performed for 20 minutes in a cooker. Tissues were blocked and then probed with a Blood Group A Antigen monoclonal antibody (Product # MA1-19693) at a dilution of 1:200 for 1 hour at room temperature. Tissues were washed extensively with TBST. Detection was performed using an HRP-conjugated anti-mouse IgG secondary detection reagent for 30 minutes, followed by DAB substrate. Tissues were counterstained with hematoxylin and visualized by light microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Mixture of erythrocytes from saliva of secretors with blood group A.|
|Storage buffer||tissue culture supernatant|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1 and 2, A antigens with chain types 3, 4, 5, 6) and Forssman antigen.
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Blood group type antigens in pancreatic intraductal papillary mucinous neoplasms.
MA1-19693 was used in immunohistochemistry to study the clinical predictive value of blood group type and blood group antigens in pancreatic intraductal papillary mucinous neoplasms
|Handra-Luca A||Hepatobiliary & pancreatic diseases international : HBPD INT (13:74)||2014|