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|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mucin from ovarian cyst fluid.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
For IHC (P), heat mediated antigen retrieval in sodium citrate is recommended.
The antibody 7LE recognizes Lewis^a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis^a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.
MA1-19344 was used in ELISA to study the specificity of a range of antiglycan antibodies
|Manimala JC,Roach TA,Li Z,Gildersleeve JC||Glycobiology (17:17C)||2007|
alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group); fucosyltransferase III; Lewis FT
CD174; FT3B; FucT-III; FUT3; LE; Les