|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Bovine|
|Host / Isotype||Yeast|
|Storage conditions||4° C|
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity was determined by bovine neutrophil chemotactic response to bovine IL-8. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically < 15.7% total responding cells at the concentration of Bovine IL-8 at 50 ng/mL.
Expression: The mature form of bovine IL-8 was expressed in Pichia pastoris, X-33. GenBank® Accession # P79255, amino acids 23-101. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: MSTELRCQCI.
Reconstitute with 0.5 ml of distilled water.
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
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Protein Aliases: Bovine Interleukin 8 IL8; C-X-C motif chemokine 8; interleukin-8; neutrophil activating peptide 1
Gene Aliases: BOS_6776; IL-8
UniProt ID: (Bovine) P79255
Entrez Gene ID: (Bovine) 280828
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