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Immunofluorescent analysis of U2OS cells expressing BPV E2 protein using a Bovine Papilloma Virus 1 E2 monoclonal antibody (Product # MA1-71537) (green) at a dilution of 0.5µg/ml followed by detection using a fluorescent 488/460 conjugated secondary antibody. Nuclei were stained using DAPI (blue).
|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length BPV type 1 E2 protein|
|Storage buffer||PBS with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.8-2 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with BPV type 1 E2 protein and has been mapped to amino acids 310-410.
This is a conformational antibody and does not work in Western blot analysis or under other denaturating conditions.
Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. 100 different HPV types have been identified to date. Various types of HPV may cause warts while others may cause a subclinical infection resulting in precancerous lesions. All HPVs are transmitted by skin-to-skin contact.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.